By Bernard N. Fields and Rudolf Jaenisch (Eds.)
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14, 327. 12. , and Rapp, F. (1973). Virology 55, 53. 13. , and Rapp, F. (1976). Science 192, 1134. 14. Rigby, P. W. , Rhodes, C , and Berg, P. (1977). J. Mol. Biol. 113, 237. 15. Southern, E. M. (1975). J. Mol. Biol. 98, 503. 16. Chang, A. C. , and Cohen, S. N. (1978). J. Bact. 134, 1141. 17. Kushner, S. R. (1978). In "Genetic Engineering" (H. W. Boyer and S. ), pp. 17-23. North Holland Biomédical Press. 18. , and Helinski, D. R. (1980). Methods in Enzymology, in press. 19. Roizman, B. (1979). Cell 16, 481.
Approximately 30% of the clones screened contained extremely small inserts (Mr of less than 1 X 1 0 6 ) . At this point, we do not know if these inserts are legitimate HCMV Eco RI fragments or products of a recombination event within the plasmid. We have yet to determine the exact size of the inserts and the number of sequences represented in this size class. Experiments are in progress to further characterize these clones. Of the 7 bands not represented among our clones, at least two must represent the ends of the genome and cannot be cloned using the present method because only one terminus of the viral fragment will have an Eco RI site.
1974) J_. Mol. Biol. 90:20-25. 12. A. Jr. (1975) J. Mol. Biol. 9L· 439-462. 13. M. (1975) J. Mol. Biol. 9L· 421-438. 14. M. S. and Fox, C F . A. Benjamin, pp. 363-384. 15. M. , and Botstein, D. (1975) J_. Mol. Biol. 98_: 413-424. 16. , Langenaur, C , and Agabian, N. (1976) J_. Virol. 17: 568-575. MOLECULAR CLONING OF THE HUMAN CYTOMEGALOVIRUS GENOME (STRAIN AD169) 1 Joyce C. Tamashiro and Deborah H. Spector Department of Biology, C-016; University of California, San Diego; La Jolla, CA 92093 ABSTRACT Human Cytomegalovirus (HCMV), one of the herpesviruses, is medically significant both as a cause of birth defects and as a source of problems in immunosuppressed individuals.