By M. Galimand, C. Vieille, B. Perroud, I. Onyeocha, C. Elmerich (auth.), Prof. Dr. Walter Klingmüller (eds.)
th th On June 17 and 18 1987 the fourth workshop on "Azospi rillum: Genetics, body structure, Ecology" came about on the Uni ver sity of Bayreuth, West Germany, prepared through the Genetics go away ment. there have been approximately eighty individuals, who got here from German learn associations, from different ecu international locations, from Is rael, Egypt, India, North Vietnam, and North and South the USA. the previous workshops had additionally taken position in Bayreuth, in 1981, 1983 and 1985, therefore the organizers may draw at the adventure received at those past workshops. Azospirilla have, in the past 15 years, chanced on an ever expanding medical curiosity simply because, first, those soil bacte ria hold the genetic details for binding molecular nitrogen from the air and, moment, they dwell in shut area to the roots of grain vegetation and forage grasses. by means of exploitation of those homes, it's was hoping to boost inoculation methods which might be utilized in nitrogen-deficient soils.
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Extra resources for Azospirillum IV: Genetics · Physiology · Ecology Proceedings of the Fourth Bayreuth Azospirillum Workshop
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15 kb EcoRI DNA fragments of AAb2c (4) as probes. 4. DNA isolation, restrietion endonuclease digestion and hybrid~zation: ~. brasilense DNA was prepared by the method of Marmur (7) . Plasmid DNA was prepared by the cleared using Triton X-IOO. Bacteriophages trifugation on a CsCl step gradient described by Maniatis et al. lysate were purified and the DNA (8). method by cenisolated as DNA was digested withre- striction endonucleases according to the instructions of the suppliers. The DNAfragments were separated by eletrophoresis on agarose gels and then transferred to nitrocellulose or nylon membranes (9).