By Jennifer J. Kohler, Visit Amazon's Steven M. Patrie Page, search results, Learn about Author Central, Steven M. Patrie,
Glycosylation is the main ample post-translational amendment of proteins. Estimates fluctuate commonly, yet a typical review is that upwards of fifty% of eukaryotic proteins are transformed through a few kind of glycan. In Mass Spectrometry of Glycoproteins: equipment and Protocols, expert researchers within the box aspect a number of the tools which are now prevalent for glycoproteomics. those equipment and methods comprise strong pattern coaching thoughts; complicated chromatographic recommendations for bettering dynamic variety; state of the art mass spectrometry instrumentation and linked ionization and fragmentation tools; and informatics instruments used for determining glycoproteins and characterizing the linked glycans. Written within the hugely profitable Methods in Molecular Biology™ sequence structure, chapters comprise introductions to their respective themes, lists of the mandatory fabrics and reagents, step by step, conveniently reproducible laboratory protocols, and key tips about troubleshooting and keeping off recognized pitfalls.
Authoritative and useful, Mass Spectrometry of Glycoproteins: tools and Protocol is a necessary source if you happen to paintings on the interface of glycobiology and mass spectrometry.